Meerkat

This site hosts the Meerkat software developed in Peter Park's lab at CBMI, Harvard Medical School. The Meerkat methodology is documented in
Diverse mechanisms of somatic structural variations in human cancer genomes
Lixing Yang1, Lovelace J. Luquette1, Nils Gehlenborg1,3, Ruibin Xi1,4, Psalm S. Haseley1,5, Chih-Heng Hsieh6, Chengsheng Zhang6, Xiaojia Ren5, Alexei Protopopov7, Lynda Chin7, Raju Kucherlapati2,5, Charles Lee6,8, Peter J. Park1,5,9,*
1Center for Biomedical Informatics, Harvard Medical School, Boston, MA 02115, USA
2Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
3Cancer Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
4School of Mathematical Sciences and Center for Statistical Science, Peking University, Beijing 100871, China
5Division of Genetics, Brigham and Women's Hospital, Boston, MA 02115, USA
6Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA
7Department of Genomic Medicine and Institute for Applied Cancer Science, MD Anderson Center, Houston, TX 77054, USA
8Department of Medicine, Seoul National University College of Medicine, Seoul 110-799, South Korea
Houston, Texas, USA
9Informatics Program, Children's Hospital, Boston, MA 02115, USA
* To whom correspondence should be addressed
Cell. 153: 919-929.

Download Meerkat

Meerkat can be downloaded through the links below. For further instructions on installing and configuring Meerkat as well as required software for running the pipeline, please see the manual included in the source distribution.

Version 0.185 .tar.gz (556KB) md5sum: 6f4fe1eabfc009e9f110dea64e5f3ae8

Demo dataset

The following tarball contains a small dataset for demonstration and to test your Meerkat installation.

Example dataset .tar.gz (156MB) md5sum: b274ea762038a6d9c9cea2d8999be066

The Meerkat Workflow

The following diagram outlines the steps taken by Meerkat to identify structural variations. Please refer to the publication for a more detailed treatment of the process.
Pink and light blue lines denote two segments of the genome connected together in the formation of an SV. In Step 3, purple lines denote repetitive segments. Arrows connected by dashed lines represent paired-end reads. In Step 4, the dark blue line on the reference genome represents a deleted segment in the donor genome; the dark red line represents an inserted segment. Light and dark green arrows connected by dashed lines represent 2 clusters of discordant read pairs. In Step 5, the beginning and end of soft-clipped and unmapped reads identified in Step 1 are used as split reads.


Frequently Asked Questions

0. I have a problem, is there documentation available?

Yes! A manual is distributed with Meerkat. Before contacting us for help, please be sure to read the manual first.

1. What if I can't get Meerkat to work on my server?

Please use the latest version of Meerkat. Meerkat requires a number of programs, modules, and references, make sure you have all of them in place and the parameters are given properly. The most likely error is some parameters are not specified correctly. If you have difficulty installing any programs, modules or references, please first contact your system admin. If you need further assistance, please contact us at ylixing aatt gmail ddoott com.

When you contact us, please provide the following: Are you able to run ./bin/bamreader from command?
Are you able to run example.bam?
The output of "ls -l" for run folder.
pre.log file if it's generated.
isinfo file if it's generated.
dre.log file if it's generated.
Error message if there is any.

2. The breakpoints are not properly annotated with fusions.pl script

The refGene.txt downloaded from UCSC needs to be sorted by chromosome and coordinate by following command:

sort refGene.txt -k 3,3 -k 5,5n > refGene_sorted.txt