H3R17me2a (multiple)

Source Catalogue # Lot # Host Species Clonality Website Validations?
W: Western blot validations
D: Dot blot validations
C: ChIP-chip/seq validataions
 Passed / Total?
  • Western blot: Pass if the histone band constituted at least 50% of the total nuclear signal, was at least ten-fold more intense than any other single nuclear band, and was at least ten-fold more intense than recombinant, unmodified histone.
  • Dot blot: Pass if at least 75% of the total signal was specific to the cognate peptide.
  • ChIP-chip/seq: Pass if the Pearson correlation is more than 0.8 on any pair of ChIPs performed from independent preparations matched for stage, cell type or biological tissue
Diagenode CS-092-100 A81-001 rabbit monoclonal Website
W2D10
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Validations

Category Species Result Notes Conditions Image Validator
Western blot fruit fly Failed Binds recombinant histone 1:125 SNAP-id open Gary Karpen Lab
Western blot human Histone + other bands 1:2000 ON at 4C open Bing Ren Lab
Dot blot Not Specific (60.2%); Cross-reacting peptides: H3K18ac (35%), H3R17me2s (4.8%) 1:2000 ON at 4C open Bing Ren Lab