Thomas Jenuwein Lab

Targets (2)

Name Target Species Antibodies Validated Species Validations?
W: Western blot validations
D: Dot blot validations
C: ChIP-chip/seq validataions
Passed / Total?
  • Western blot: Pass if the histone band constituted at least 50% of the total nuclear signal, was at least ten-fold more intense than any other single nuclear band, and was at least ten-fold more intense than recombinant, unmodified histone.
  • Dot blot: Pass if at least 75% of the total signal was specific to the cognate peptide.
  • ChIP-chip/seq: Pass if the Pearson correlation is more than 0.8 on any pair of ChIPs performed from independent preparations matched for stage, cell type or biological tissue
H3K27me1 multiple 8 human, multiple, worm
W7D8C5
11 / 20Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3K27me2 multiple 9 fruit fly, human, multiple, worm
W6D6C4
5 / 16Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4