Vincenzo Pirrotta Lab

Targets (13)

Name Target Species Antibodies Validated Species Validations?
W: Western blot validations
D: Dot blot validations
C: ChIP-chip/seq validataions
Passed / Total?
  • Western blot: Pass if the histone band constituted at least 50% of the total nuclear signal, was at least ten-fold more intense than any other single nuclear band, and was at least ten-fold more intense than recombinant, unmodified histone.
  • Dot blot: Pass if at least 75% of the total signal was specific to the cognate peptide.
  • ChIP-chip/seq: Pass if the Pearson correlation is more than 0.8 on any pair of ChIPs performed from independent preparations matched for stage, cell type or biological tissue
H3K27ac multiple 9 fruit fly, human, worm
W9D6C6
14 / 21Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3K27me1 multiple 8 human, multiple, worm
W7D8C5
11 / 20Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3K27me2 multiple 9 fruit fly, human, multiple, worm
W6D6C4
5 / 16Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3K27me3 multiple 11 fruit fly, human, multiple, worm
W10D7C8
15 / 25Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3K4me1 multiple 8 fruit fly, human, multiple, worm
W8D7C8
17 / 23Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3K4me2 multiple 8 fruit fly, human, worm
W8D7C6
17 / 21Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3K4me3 multiple 10 fruit fly, human, worm
W8D8C9
22 / 25Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3K79me1 multiple 4 fruit fly, human, worm
W5D4C5
9 / 14Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3K79me2 multiple 5 fruit fly, human, worm
W4D4C4
10 / 12Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3K79me3 multiple 4 fruit fly, human, multiple, worm
W3D4C2
4 / 9Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3S10ph multiple 8 fruit fly, human, worm
W8D4C2
7 / 14Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H3S28ph multiple 5 fruit fly, worm
W50C2
3 / 7Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H4ac4 multiple 2 fruit fly, worm
W10C2
3 / 3Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4