H3K27me1 (multiple)

Source Catalogue # Lot # Host Species Clonality Validations?
W: Western blot validations
D: Dot blot validations
C: ChIP-chip/seq validataions
 Passed / Total?
  • Western blot: Pass if the histone band constituted at least 50% of the total nuclear signal, was at least ten-fold more intense than any other single nuclear band, and was at least ten-fold more intense than recombinant, unmodified histone.
  • Dot blot: Pass if at least 75% of the total signal was specific to the cognate peptide.
  • ChIP-chip/seq: Pass if the Pearson correlation is more than 0.8 on any pair of ChIPs performed from independent preparations matched for stage, cell type or biological tissue
Thomas Jenuwein Lab Rabbit 8835 rabbit polyclonal
W1D2C1
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Validations

Category Species Result Notes Conditions Image Validator
Western blot worm Passed Single histone band 1:1000 of serum ON at 4C open Strome Lab
Dot blot Specific (13%); Cross-reacting peptides: H3K27me2 (13%) 1:5000, 1 hour, RT open Vincenzo Pirrotta Lab
Dot blot multiple Passed Specific (~75%); cross-reacting peptides: H3K27me2 (10%), H3K27me3 (15%), H4K20me1 (<1%) 1:5000 ON 4C open Ahringer Lab
ChIP-chip/seq worm Passed Strome and Ahringer Lab