H3K18ac (multiple)

Source Catalogue # Lot # Host Species Clonality Website Validations?
W: Western blot validations
D: Dot blot validations
C: ChIP-chip/seq validataions
 Passed / Total?
  • Western blot: Pass if the histone band constituted at least 50% of the total nuclear signal, was at least ten-fold more intense than any other single nuclear band, and was at least ten-fold more intense than recombinant, unmodified histone.
  • Dot blot: Pass if at least 75% of the total signal was specific to the cognate peptide.
  • ChIP-chip/seq: Pass if the Pearson correlation is more than 0.8 on any pair of ChIPs performed from independent preparations matched for stage, cell type or biological tissue
Abcam ab1191 GR31152-1 multiple polyclonal Website
0D20
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Validations

Category Species Result Notes Conditions Image Validator
Dot blot multiple Passed Specific (>90%); cross-reacting peptides: H3R17me2 asym (<10%) 1:4000 ON at 4C open Ahringer Lab
Dot blot multiple Passed Specific (>90%); cross-reacting peptides: H3R17me2 (asym) (<10%) 1:4000 ON 4C open Ahringer Lab