H3K27me2 (multiple)

Source Catalogue # Lot # Host Species Clonality Website Validations?
W: Western blot validations
D: Dot blot validations
C: ChIP-chip/seq validataions
 Passed / Total?
  • Western blot: Pass if the histone band constituted at least 50% of the total nuclear signal, was at least ten-fold more intense than any other single nuclear band, and was at least ten-fold more intense than recombinant, unmodified histone.
  • Dot blot: Pass if at least 75% of the total signal was specific to the cognate peptide.
  • ChIP-chip/seq: Pass if the Pearson correlation is more than 0.8 on any pair of ChIPs performed from independent preparations matched for stage, cell type or biological tissue
Abcam ab24684 614458 rabbit polyclonal Website
W1D10
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Validations

Category Species Result Notes Conditions Image Validator
Western blot human Histone + other bands 0.5 ug/ml ON at 4C open Bing Ren Lab
Dot blot Not Specific (36.5%); Cross-reacting peptides: H3K27me1 (42.2%), H3K27me3 (14.3%), H1.2 (6.6%), H3K9me2 (0.2%), H3K9me1 (0.1%), H3K9ac (0.1%) 0.5 ug/ml ON at 4C open Bing Ren Lab