H3K36me2 (multiple)

Source Catalogue # Lot # Host Species Clonality Website Validations?
W: Western blot validations
D: Dot blot validations
C: ChIP-chip/seq validataions
 Passed / Total?
  • Western blot: Pass if the histone band constituted at least 50% of the total nuclear signal, was at least ten-fold more intense than any other single nuclear band, and was at least ten-fold more intense than recombinant, unmodified histone.
  • Dot blot: Pass if at least 75% of the total signal was specific to the cognate peptide.
  • ChIP-chip/seq: Pass if the Pearson correlation is more than 0.8 on any pair of ChIPs performed from independent preparations matched for stage, cell type or biological tissue
Diagenode CS-127-100 A239-001 rabbit polyclonal Website
W1D10
 0 / 2Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4

Validations

Category Species Result Notes Conditions Image Validator
Western blot human Histone + other bands 1:2000 ON at 4C open Bing Ren Lab
Dot blot Not Specific (61.7%); Cross-reacting peptides: H3K36me3 (33.5%), H3R17me2a (2.2%), H3K36me1 (2.1%), H3K18ac (0.4%), H3K4me2 (0.1%) 1:2000 ON at 4C open Bing Ren Lab