Vincenzo Pirrotta Lab

Targets (13)

Name	Target Species	Antibodies	Validated Species	Validations? W: Western blot validations D: Dot blot validations C: ChIP-chip/seq validataions	Passed / Total? Western blot: Pass if the histone band constituted at least 50% of the total nuclear signal, was at least ten-fold more intense than any other single nuclear band, and was at least ten-fold more intense than recombinant, unmodified histone. Dot blot: Pass if at least 75% of the total signal was specific to the cognate peptide. ChIP-chip/seq: Pass if the Pearson correlation is more than 0.8 on any pair of ChIPs performed from independent preparations matched for stage, cell type or biological tissue
H3K27ac	multiple	9	fruit fly, human, worm	W9D6C6	14 / 21
H3K27me1	multiple	8	human, multiple, worm	W7D8C5	11 / 20
H3K27me2	multiple	9	fruit fly, human, multiple, worm	W6D6C4	5 / 16
H3K27me3	multiple	11	fruit fly, human, multiple, worm	W10D7C8	15 / 25
H3K4me1	multiple	8	fruit fly, human, multiple, worm	W8D7C8	17 / 23
H3K4me2	multiple	8	fruit fly, human, worm	W8D7C6	17 / 21
H3K4me3	multiple	10	fruit fly, human, worm	W8D8C9	22 / 25
H3K79me1	multiple	4	fruit fly, human, worm	W5D4C5	9 / 14
H3K79me2	multiple	5	fruit fly, human, worm	W4D4C4	10 / 12
H3K79me3	multiple	4	fruit fly, human, multiple, worm	W3D4C2	4 / 9
H3S10ph	multiple	8	fruit fly, human, worm	W8D4C2	7 / 14
H3S28ph	multiple	5	fruit fly, worm	W50C2	3 / 7
H4ac4	multiple	2	fruit fly, worm	W10C2	3 / 3