Julie Ahringer Lab

Targets (2)

Name Target Species Antibodies Validated Species Validations?
W: Western blot validations
D: Dot blot validations
C: ChIP-chip/seq validataions
Passed / Total?
  • Western blot: Pass if the histone band constituted at least 50% of the total nuclear signal, was at least ten-fold more intense than any other single nuclear band, and was at least ten-fold more intense than recombinant, unmodified histone.
  • Dot blot: Pass if at least 75% of the total signal was specific to the cognate peptide.
  • ChIP-chip/seq: Pass if the Pearson correlation is more than 0.8 on any pair of ChIPs performed from independent preparations matched for stage, cell type or biological tissue
H3K9me3 multiple 16 fruit fly, human, multiple, worm
W15D10C10
25 / 35Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4
H4 multiple 3 fruit fly, worm
W20C1
3 / 3Chart?chs=36x36&cht=p&chco=008000|eeeeee&chf=bg,s,ffffff00&chp=4