Gary Karpen Lab

Targets (9)

Name	Target Species	Antibodies	Validated Species	Validations? W: Western blot validations D: Dot blot validations C: ChIP-chip/seq validataions	Passed / Total? Western blot: Pass if the histone band constituted at least 50% of the total nuclear signal, was at least ten-fold more intense than any other single nuclear band, and was at least ten-fold more intense than recombinant, unmodified histone. Dot blot: Pass if at least 75% of the total signal was specific to the cognate peptide. ChIP-chip/seq: Pass if the Pearson correlation is more than 0.8 on any pair of ChIPs performed from independent preparations matched for stage, cell type or biological tissue
CitH3	multiple	1	fruit fly	W100	0 / 1
H3K56ac	multiple	3	fruit fly, human	W3D2C2	4 / 7
H3R17me2a	multiple	5	fruit fly, human	W6D3C1	1 / 10
H3R2me1	multiple	1	fruit fly, human	W2D10	0 / 3
H4	multiple	3	fruit fly, worm	W20C1	3 / 3
H4K20me1	multiple	9	fruit fly, human, multiple, worm	W7D8C4	17 / 19
H4K20me2	multiple	3	fruit fly, worm	W2D10	2 / 3
H4K20me3	multiple	11	fruit fly, human, multiple, worm	W11D7C4	3 / 22
H4R3me2	multiple	2	fruit fly	W200	0 / 2